ZmMATE6 from maize encodes a citrate transporter that enhances aluminum tolerance in transgenic Arabidopsis thaliana

The yields of cereal crops grown on acidic soils are often reduced by aluminum (Al) toxicity because the prevalence toxic Al3+ cations increases as pH falls below 5.0. Al-dependent release citrate from resistant lines maize is controlled ZmMATE1 which encodes a multidrug and compound extrusion (MATE) transporter protein. ZmMATE6 another member this family in whose expression also increased Al treatment. We investigated function gene more detail to determine whether it contributes resistance. Quantitative RT-PCR measurements found that was expressed roots leaves Al-resistant sensitive inbred lines. Treatment with induced all tissues but several other divalent or trivalent tested had no effect expression. This pattern induction treatment confirmed promoter–?-glucuronidase fusion Heterogeneous displayed greater Al-activated significantly than controls. associated accumulation root tissues. Our results demonstrated functions transporter.